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Applications of Biotechnology

Type II restr...

Question

Type II restriction endonuclease cleave DNA at?

A

Random sequence

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B

Specific DNA sequence

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C

Single stranded sequence

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D

None of the above

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Solution

The correct option is B Specific DNA sequence
Type ll restriction endonuclese has specific recognition sites on the DNA sequence . So they cleave the DNA only at that specific sites. So the correct option is B.

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Similar questions

Q. Assertion :Restriction endonuclease recognize short palindromic sequence and cut at specific sites. Reason: When a restriction endonuclease acts on palindrome, it cleaves both the strands of DNA molecule.

Q. Restriction endonucleases are enzymes that cleave DNA molecules into smaller fragments. Which type of bond do they act on?

Q. The restriction endonuclease enzymes cut the DNA strands at specific sequences known as ______.

Q. Which of the following would be required for the synthesis of rDNA?
(I) Restriction endonuclease
(II) DNA fragments
(III) DNA ligase
(IV) DNA helicase

Q. The restriction endonuclease EcoRI recognises and cleaves DNA sequence as shown below :
5' - GAATTC - 3'
3' - CTTAAG - 5'
What is the probable number of cleavage sites that can occur in a 10 kb long random DNA sequence?

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