The correct option is B Closure of bicuspid and tricuspid valves followed by semi-lunar valves
The 'lub' is the first heart sound, commonly termed as S1 and is caused by the turbulence caused by the closure of the mitral (or bicuspid) and tricuspid valves at the start of systole. The second sound 'dub' or S2 is caused by the closure of aortic and pulmonic (Semi-lunar) valves, marking the end of systole. Thus, the correct answer is option (C), 'Closure of bicuspid and tricuspid valves followed by semi-lunar valves'.