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When E.coli is cultured in a medium containing antibiotic, only cells containing recombinant DNA will be able to survive due to antibiotic resistance genes and one will be able to isolate the recombinants.
Explain it.
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Solution

Antibiotic resistant markers are the most commonly used selection markers which are used to detect recombinant bacteria and separate them from non recombinant bacteria.

Most commonly used plasmid vectors contain antibiotic resistant genes as selection markers. During the cloning procedure the DNA of interest is ligated to the plasmid vector and inserted into the bacterium. Then to isolate the recombinant bacteria, antibiotic containing media is used. Recombinant bacteria will grow on that media as they contain the antibiotic resistant gene. But non recombinant bacteria will not grow as the antibiotic will kill those cells. So, one can easily isolate the recombinants from the colonies growing on antibiotic containing media plates.

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Q. If a recombinant DNA bearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the non-transformed recipient cells will die. The ampicillin-resistant gene in this case is called as _______________.

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rnrnWhen E.coli is cultured in a medium containing antibiotic, only cells containing recombinant DNA will be able to survive due to antibiotic resistance genes and one will be able to isolate the recombinants.Explain it.rn rn

rn
rn
When E.coli is cultured in a medium containing antibiotic, only cells containing recombinant DNA will be able to survive due to antibiotic resistance genes and one will be able to isolate the recombinants.
Explain it.
rn

rn