Question

Ultrasonic sound waves are used in which technique of cytology?

• A. X-ray diffraction
• B. Chromatography
• C. Cell fractionation
• D. Autoradiography

Answer 1

The correct option is C Cell fractionation

Cell fractionation is the separation of homogeneous sets from a larger population of cells. Tissue is typically homogenized in an isotonic buffer solution, as well as a pH buffer by use of a variety of mechanisms such as grinding, mincing, chopping, pressure changes, osmotic shock, freeze-thawing and ultrasound homogenization. This is done to stop osmotic damage. The samples are then kept cold to prevent enzymatic damage.

An autoradiograph is an image on an x-ray film or nuclear emulsion produced by the pattern of decay emissions (e.g., $\beta$-particles or $\gamma$-rays) from a distribution of a radioactive substance.

Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate.

X-ray diffraction does not use ultrasonic sounds.