Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as an adaptation. These imprints need to be used
Esther and Joshua hypothesized that antibiotic resistant strains of bacteria surviving an application of antibiotics had the resistance before their exposure to the antibiotics, not as a result of the exposure. So the Streptomycin-resistant bacteria were there in the population before they encountered streptomycin. They did not evolve resistance in response to exposure to the antibiotic. And they proved this using imprints from a plate with complete medium carrying bacterial colonies and these imprints need to be used on plates with streptomycin only.