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Question

Vmax is best defined as:


A

Highest possible concentration of the substrate at which the reaction rate will be the highest

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B

Highest possible substrate concentration that can bind to the enzymes

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C

The point at which the reaction is saturated

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D

All of the above

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Solution

The correct options are
A

Highest possible concentration of the substrate at which the reaction rate will be the highest


B

Highest possible substrate concentration that can bind to the enzymes


C

The point at which the reaction is saturated


Vmax or maximum velocity of an enzymatic reaction can be defined as the rate of the reaction at which the enzyme shows highest turnover. Increasing the substrate concentration indefinitely does not increase the rate of an enzyme-catalyzed reaction beyond a certain point. This point is reached when there are enough substrate molecules to completely fill (saturate) the enzyme's active sites. Active site is the substrate binding - reaction site of the enzyme. The maximal velocity, or Vmax, is the rate of the reaction under these conditions. Vmax reflects how fast the enzyme can catalyze the reaction.


Vmax is not the substrate concentration.

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