Question

VNTR is employed for

• A. Protoplasm culture
• B. DNA finger printing
• C. Regulation of plant growth hormones
• D. Enhancing photosynthesis in desert plant

Answer 1

The correct option is B DNA finger printing

A variable number tandem repeat (VNTR) refers to the presence of a short nucleotide sequence as a tandem repeat on many chromosomes; it exhibits variations in length and number of repeats between individuals. VNTR are 10-100 bp long. This DNA polymorphism is revealed during DNA finger printing in creating DNA profile of individuals. For the purpose, restriction enzyme digested DNA samples are sorted by gel electrophoresis followed by southern blotting. Finally, radioactive probes are washed over the nylon surface to allow their joining to any DNA fragments of the same composition; option B is correct.

Protoplasm culture is the fusion of protoplasm of two different species /individuals to bring their traits together in the hybrid.

Plant growth hormones are the natural as well as artificially synthesized chemicals that control plant growth and development. Genes for abiotic stress tolerance factors such as glycine betaine are cloned in desert plants to increase their photosynthetic capacity by enhancing their tolerance for abiotic stress. The correct answer is B.