we use alkaline phosphatase to remove phosphate group to prevent unwanted internal binding .so why don't we use any enzyme which can remove OHgroup from 3'end

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Why do we separate substances present in a mixture?
i) To separate wanted components from unwanted components.
ii) To remove impurities.

Why do we separate substances in a mixture?

i) To separate two different but useful components.
ii) To remove useless components.
iii) To remove impurities.

Separation process	Purpose for which we do the separation	What do we do with the separated components
1) Separate stones from rice	a) To remove non useful components	i) We throw away the impurities
2) Churning milk to obtain butter	b) To remove impurities or harmful components	ii) we throw away the solid component
3) Separate tea leaves	c) to separate two different but useful components.	iii) we use both the components.

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