Question

What additional treatment is required for protoplast fusion in plants?

• A. Polyethylene glycol and sodium nitrate
• B. Coconut milk and glycine
• C. Cellulase and pectinase
• D. All the above

Answer 1

The correct option is C Cellulase and pectinase

The process of obtaining plant by the culture of isolated protoplast from the plant parts on an artificial media of known composition is known as protoplast culture. In order to culture the protoplast, it is isolated from the explant. The protoplast is enclosed by the cell wall and plasma membrane in a plant cell so enzyme cellulase and pectinase are used to digest the cell wall which is composed of cellulose, hemicellulose and pectin.

So, the correct answer is option C.