Question

What approaches can be employed for virus isolation?

Answer 1

Virus isolation:

1. The process of virus purification uses a lot of properties of viruses.
2. Virions are larger compared to proteins and usually are stable compared to components of normal cells, it has surface proteins.
3. Several techniques to isolate organelles and proteins are used in the isolation of viruses.
4. The most widely used approaches are –
   1. Precipitation of viruses.
   2. Density and differential gradient centrifugation.
   3. Denaturation of contaminants.
   4. Cell constituents are digested by the action of enzymes.