Question

What are genomic and cDNA libraries and how can they be prepared ? What is their advantage?

Answer 1

A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA.

A cDNA library represents a collection of only the genes that are encoded into proteins by an organism. Complementary DNA, or cDNA, is created through reverse transcription of messenger RNA, and a library of cDNAs is generated using DNA cloning technology.

Genomic and cDNA library help in:

• Determining the complete genome sequence of a given organism.
• Serving as a source of genomic sequence for the generation of transgenic animals through genetic engineering.
• Study of the function of regulatory sequences in vitro.
• Study of genetic mutations in cancer tissues.