Question

What are the basic components of PCR?

Answer 1

PCR technique:

1. The PCR( polymerase chain reaction) refers to a widely used method to make large copies of specific DNA samples rapidly.
2. The components that are required for PCR:

1. DNA templates- DNA templates are the DNA segments containing the desired target sequences that would be amplified.
2. Primers- The primer pairs usually help in hybridizing the sample DNA and recognizing the amplification region.
3. DNA polymerase- DNA polymerase starts the replication process.
4. dNTPs- In PCR, dNTPs are used as the building blocks of newly synthesized DNA.
5. buffer/cofactors- Buffer is used in PCR to ensure an optimal condition during the process.