Question

What are the characteristics of restriction enzymes?

Answer 1

Restriction enzymes:

1. They are nucleases in nature.
2. They cleave both polynucleotide strands of DNA at a specific sequence of nitrogenous bases.
3. These sequences form recognition sites, restriction sites, or target sites.
4. These sites have palindromic sequences of nitrogenous bases.
5. Each palindrome has a specific endonuclease.
6. EcoRI is a restriction endonuclease that is found in Escherichia coli that recognizes a palindromic sequence GAATTC in both strands of DNA in the 5'-3' direction.