Question

What are the disadvantages of ELISA?

Answer 1

ELISA:

1. ELISA or Enzyme-Linked Immunosorbent Assay is an immunoassay technique utilized to detect diseases.
2. It is a plate-based technique designed to detect and quantify soluble substances such as proteins, hormones, peptides, and antibodies.

ELISA's disadvantages are as follows:

1. Inadequate inhibition of immunogenic antigen in ELISA results in false results.
2. Refrigerated transport and storage are necessary because antibodies are proteins.
3. ELISA is labor-intensive and costly to prepare antibodies.
4. There is a high probability of false-positive and negative within it.
5. Instability is found in ELISA antibodies.