Question

What are the steps through which the process of immunoprecipitation is carried out?

Answer 1

Immunoprecipitation:

1. Immunoprecipitation is a technique for isolating a particular antigen from a mixture via the antigen-antibody interaction.
2. IP, SDS-PAGE, or Western blotting techniques are utilized to isolate antigens.
3. Sample preparation, pre-clearing, antibody incubation/formation of antibody-antigen complexes, precipitation, and washing are the five key phases in the immunoprecipitation assay procedure.
4. Types: Immunoprecipitation of individual proteins (IP), Immunoprecipitation of protein complexes (Co-IP), Immunoprecipitation of chromatin (ChIP), Immunoprecipitation of RNPs (RIP) Proteins with tags.