Question

What are the properties of ideal cloning vectors?

Answer 1

Characteristics of a cloning vectors

1. it must be small in size
   
2. It must be self-replicating inside host cell
   
3. It must possess restriction site for Restriction Endonuclease enzymes
   
4. Introduction of donor DNA fragment must not interfere with replication property of the vector
   
5. It must possess some marker gene such that it can be used for later identification of recombinant cell
   
6. it must possess multiple cloning site
   

Plasmid (PBR322):