Question

What are the steps of DNA fingerprinting?

Answer 1

DNA fingerprinting:

1. It is a chemical test that reveals a person's or other living thing's genetic make-up.
2. It is a technology that identifies several minisatellites in the genome at the same time to form a pattern that is unique to an individual.
3. It is used as evidence in court, to identify remains, locate blood relations, and search for illness remedies.
4. Alec Jeffreys invented this approach, in which he employed satellite DNAs, also known as VNTRs (Variable Number of Tandem Repeats), as a probe since they revealed a high amount of polymorphism.

Steps involved in DNA fingerprinting:

1. DNA extraction
2. Digesting the DNA with restriction endonuclease enzymes
3. Using electrophoresis, separate the digested fragments based on fragment size.
4. Blotting isolated particles onto synthetic membranes such as nylon
5. Use tagged VNTR probes to hybridize the fragments
6. Using autoradiography to examine the hybrid pieces