Question

What bonds do restriction enzymes break?

Answer 1

Restriction enzymes:

1. The restriction enzyme is a protein produced by the bacteria that cleaves the DNA at specific sites.
2. This site is known as restriction site.
3. The restriction enzymes also recognize short and specific nucleotide sequences in the DNA known as recognition sequences.
4. When restriction enzyme recognizes a DNA sequence, it hydrolyzes the bond between adjacent nucleotide & cuts through the DNA molecule.
5. Restriction enzymes break phosphodiester bonds between the phosphate and the pentose sugar in sugar-phosphate backbone at the specific site.