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Question

What does pBR322 stand for?

A
Plasmid bearing 322 restriction sites
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B
Plasmid made by Boyer and Rodriguez.
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C
Plasmid made by Bolivar and Rodriguez
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D
Plasmid containing just 322 nucleotides.
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Solution

The correct option is C Plasmid made by Bolivar and Rodriguez
Many plasmids that are used today have been synthesized by scientists to suit the requirements they need. pBR322 was one of the most popularly used plasmids. Bolivar and Rodriguez, two postdoctoral researchers in Herbert Boyer’s lab made many iterations of this plasmid. They numbered one of them as 322 to identify it from the rest of the plasmids they constructed. In pBR322-’p’ stands for plasmid, ‘B’ stands for Bolivar and ‘R’ stands for Rodriguez.

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