Question

What does the sensitivity of sandwich elisa depend upon?

Answer 1

ELISA:

1. It stands for enzyme-linked immunosorbent assay, it is a laboratory test to detect antibodies in the blood.
2. The principle of ELISA is antigen-antibody interaction.
3. There are four types of ELISA namely Direct ELISA (antigen-coated plate; screening antibody), Indirect ELISA (antigen-coated plate; screening antigen/antibody), Sandwich ELISA (antibody-coated plate; screening antigen), and Competitive ELISA (screening antibody)

The sensitivity of the Sandwich ELISA depends on four factors:

1. Avidity of the first antibody for the antigen.
2. Solid-phase bound with the first antibody molecules number
3. Avidity of the second antibody for the antigen.
4. The particular activity of the second antibody.