Question

What enzymes are used in DNA replication?

Answer 1

DNA replication:

1. DNA replication is a semiconservative process.
2. Several enzymes and RNA molecules are utilized to create a new complementary daughter strand from a parental strand (template) in DNA replication.

Enzymes used in DNA replication:

1. Primase (catalyze the synthesis of short RNA molecules used as primers for DNA polymerase)
2. DNA polymerase I (for filling small DNA segments during replication and repair processes)
3. DNA polymerase III (It is the main replicating enzyme)
4. Helicase (separate double-stranded DNA into single strands allowing each strand to be copied)
5. DNA gyrase (reduces supercoiling which builds up during DNA unwinding)
6. DNA ligase (Joins the discontinuously synthesized fragments)