Question

What happens in the third step of the polymerase chain reaction?

Answer 1

Polymerase chain reaction:

1. It is a technique for rapidly and precisely replicating a specified region of DNA.
2. The polymerase chain reaction allows researchers to obtain enormous amounts of DNA needed for numerous investigations and processes in molecular biology, forensic analysis, evolutionary biology, and medical diagnostics.
3. The capacity of DNA polymerase to manufacture a new strand of DNA complementary to the supplied template strand is used in PCR.

Steps of PCR:

PCR is based on three straightforward stages that are necessary for each DNA synthesis reaction:

1. Denaturation of the template into single strands;
2. Annealing of primers to each original strand for new strand synthesis
3. Extension of the primer-derived new DNA strands.

The third step of PCR:

1. A thermostable DNA polymerase is utilized in the third phase of extension.
2. Taq polymerase is a typical tool for this. It is carried out at a temperature of 75-80 °C (72°C).
3. The DNA polymerase synthesizes the complementary strand of the DNA template by adding nucleotides in the 5'-3' orientation.