What happens when two different DNA samples are snipped with a similar sticky end restriction enzyme?
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Solution
Restriction enzyme:
These are endonuclease enzymes that cleave a DNA molecule at a particular site called the restriction site.
The restriction enzyme cleaves the DNA sequence asymmetrically to produce short and single-stranded structures which are called sticky or cohesive ends because these can stick together on treatment with a ligase to form the DNA molecule again.
For example,a six nucleotide palindromic sequence recognized by the restriction enzyme EcoR1 cleaves both strands of DNA at different locations.
Thus, when two DNA samples are treated and cleaved with a similar sticky end restriction enzyme then the fragments of different lengths of DNA sequence are observed because in both DNA sequences the recognition or restriction site would be different.