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Question

What is a double thymidine block?


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Solution

Double thymidine block:

  1. During cell division, a cell crosses through various phases in order to get duplicated into two.
  2. Three such phases in order are the G1, S, and G2 phases.
  3. Thymidine is a chemical used during cell division that acts as an inhibitor of DNA synthesis.
  4. This action arrests the cell at the G1 to S boundary just before DNA replication.
  5. Another treatment with thymidine arrests all the cells at the start of the next phase i.e., the S phase.
  6. This method is called double thymidine block.
  7. This method is used to obtain synchronized cells all at a stage prior to the beginning of the S phase by treating the asynchronous cells with thymidine.

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