Question

What is the drawback of using mammalian cells as an experimental system?

Answer 1

Following are the drawbacks of using mammalian cells as an experimental system:

1. Bigger genome hence more genes to work with.
2. Makes the target gene difficult to express.
3. Requires greater time, effort, money, and resources to silence the activity of the unwanted genes.
4. Proteins undergo post-translational modification that makes studying the proteins more difficult.
5. The cell duplication rate is much lower as compared to bacteria and yeasts.