Bi-orientation is defined as the chromosomal orientation to opposite poles of the bipolar spindle before cell division.
The microtubule array should be bipolar, the chromatid pairs should be aligned on the spindle for each sister associated with an opposite spindle pole.
The chromosome attachment and spindle structure in bilateral symmetry are vital to the spindle’s ability to pull the chromatid pairs apart and move an entire chromosome set to each end of the cell.