Question

What is chromatography?

Answer 1

Chromatography

• Chromatography is a technique used to separate those solutes of a mixture that are soluble in the same solvent. It works on the principle of adsorption. It is used to separate the colored components such as(dyes) in black ink.
• Chromatography is the technique for the separation, purification, and testing of compounds.
• Chromatography is a separation method where the analyte is combined within a liquid or gaseous mobile phase., which is pumped through a stationary phase.
• Usually, one phase is hydrophilic and the other is lipophilic.
• The components of the analyte interact differently with these two phases.
• Depending on their polarity they spend more or less time interacting with the stationary phase and are thus retarded to a greater or lesser extent. This leads to the separation of the different components present in the sample.
• Each sample component elutes from the stationary phase at a specific time called the retention time.
• As the components pass through the detector their signal is recorded and plotted in the form of a chromatogram.