The correct option is B All are ex-situ conservation methods
Ex situ conservation literally means "off-site conservation".
In in-vitro fertilization, egg and sperm are fertilized outside the female body in laboratory conditions. The embryo formed can then be frozen for storage or transferred to a woman's uterus.
The storage of sperms, pollen, tissue, or embryos in very low temperature like liquid nitrogen is called cryopreservation.
Similarly, tissue culture is a technique in which small fragments of a plant (explant) are introduced into an artificial nutrient medium, which allows its functioning or growth.
Hence, in vitro fertilisation, cryopreservation and tissue culture are used in ex-situ conservation of endangered species. All these methods require less space.