Question

What is DNA fingerprinting ? Describe various steps involved in the same.

Answer 1

DNA fingerprinting is a method used to identify an individual from a sample of DNA by looking at unique patterns in their DNA. Steps involved in DNA fingerprinting are:

(i) isolation of DNA

(ii) digestion of DNA by restriction endonucleases

(iii) separation of DNA fragments by electrophoresis

(iv) transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon

(v) hybridisation using labelled VNTR probe

(vi) detection of hybridised DNA fragments by autoradiography.