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What is downstream processing?


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Downstream process:

  1. Downstream processing is the process of isolation, purification, and separation of products from recombinant DNA technology (RDT).
  2. Downstream processing is done in three steps: Removal of insoluble matter, Product isolation and purification, and Product polishing.
  3. Removal of the insoluble matter: Acquiring particulate-free liquid using filtration, density gradient centrifugation, sedimentation, and precipitation.
  4. Product isolation and purification: Removal of substances having different properties than the desired product using ultrafiltration, chromatography, solvent extraction, etc.
  5. Product polishing: Packaging of the final product.

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