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Question

What is protoplast fusion?

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Solution

Protoplast fusion:

The protoplasm of the living plant cell excluding the cell wall is called protoplast. Fusion of the protoplast derived from somatic cells of plants belonging to different species produces a hybrid protoplast, this process is called protoplast fusion. Fusion of protoplasts can be obtained -

  • Either chemically with the help of a mixture of polyethylene glycol (PEG) and a high concentration of calcium at pH 8.
  • Or physically by electrofusion.
  • The pomato is a transgenic crop produced by protoplast fusion of a tomato plant and a potato plant.

Diagram: Protoplast fusion of tomato and potato to form pomato cell.


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