Question

What is pUC plasmid?

Answer 1

pUC plasmid:

1. Joachim Messing and co-workers developed a variety of plasmid cloning vectors, including pUC19.
2. The term "pUC" is derived from the conventional "p" prefix (which stands for "plasmid") and the abbreviation for the University of California, where early work on the plasmid series was done.
3. It is used to verify transformation efficiency by controlling DNA (10 pg/l).
4. To achieve optimal transformation efficiency, use experimental DNA that is devoid of phenol, ethanol, salts, protein, and detergents.
5. The blue/white colony screen, which detects recombinant plasmids, is an important characteristic of pUC plasmids.
6. The inhibition of beta-lacZa galactosidase's peptide, which is expressed by the vector, provides the basis for this screen.