Question

What is Pulsed-field Gel Electrophoresis?

Answer 1

Pulsed-field Gel Electrophoresis:

1. The method of eliminating big deoxyribonucleic acid (DNA) molecules along a gel network that changes direction at periodic intervals using a flow of electricity is known as pulsed field gel electrophoresis (PFGE).
2. During PFGE, the electrical field between spatially different pairs of electrodes is alternated in order to resolve DNA.
3. This method isolates DNA fragments by readjusting and forcing DNA fragments through the pores of an agarose gel at varying speeds.
4. Scientists utilize a method called as pulsed-field gel electrophoresis to create a DNA fingerprint for an isolated microorganism (PFGE).
5. A cluster of bacteria from the same species is called a bacterial isolate. Pulse Net looks at isolated bacteria from sick people, spoiled food, and food processing facilities.