Question

What is the action of Biuret reagent on proteins?

Answer 1

The Biuret reagent, initially blue in color, consists of $C{u}^{+2}$ ions in an alkaline solution containing $O{H}^{-}$ ions. When mixed with a solution containing protein, it interacts with the peptide bond of proteins to form a purple colored complex known as the Biuret Complex. The reagent is thus, used in the identification of proteins.