Question

What is the application of electroporation?

Answer 1

Electroporation :

1. Electroporation is a microbiology method that includes creating transitory openings in the cell membrane in order to allow the entrance of gene constructs.
2. The purpose of this approach is to improve the permeability of the cell membrane.

Application:

1. Thousands of volts (8 kV/cm) are passed over suspended cells in an electroporation cuvette to cause electroporation.
2. Following that, the cells must be handled with care until they have had a chance to proliferate and create new cells with replicated plasmids.
3. This method is 10 times more efficient than chemical transformation in enhancing cell membrane permeability.
4. Electroporation was originally utilised in medicine to introduce poorly permeant anticancer medicines into tumour nodules.
5. Gene electro transfer quickly gained popularity because to its inexpensive cost, ease of implementation, and safety.
6. When employed for DNA transfer, viral vectors can have major limitations in terms of immunogenicity and pathogenicity.