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Question

What is the coupling effect seen in the process of NADP reduction?


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Solution

The coupling effect in the process of NADP reduction:

  1. The electron transport chain is known to traverse the inner mitochondrial membrane in eukaryotes.
  2. Electrons from NADH or NADPH and FADH2 move along the electron transport chain at the inner mitochondrial membrane to oxygen, where they convert it to water.
  3. The electron transport chain is made up of a sequence of electron donors and acceptors that contribute and take electrons until they reach the oxygen molecule, which is the chain's most electronegative and terminal acceptor of electrons.
  4. The development of a proton gradient across the intermembrane gap is caused by the flow of electrons between acceptors and donors.
  5. The phosphorylation of ADP into ATP, also known as oxidative phosphorylation, takes place in this gradient.
  6. NADP reduction results in the movement of two electrons across the inner mitochondrial membrane.
  7. This results in the formation of a proton gradient, which leads to the production of ATP.
  8. ATPase enzymes catalyze the reversal of this process, resulting in the creation of ADP and Pi. This is called the coupling effect.

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