Question

What is the difference between RT PCR and PCR?

Answer 1

RT-PCR:

One of the most sensitive ways of detecting mRNA is reverse transcription-polymerase chain reaction (RT-PCR).

PCR:

1. Polymerase Chain Reaction (PCR) is a basic but groundbreaking process.
2. PCR makes advantage of the enzyme DNA polymerase's capacity to generate new strands of DNA that are complementary to the template strand.

The difference between RT PCR and PCR:

RT PCR	PCR
1. RT-PCR is a PCR variation used in molecular biology to measure gene expression.	1. PCR is a process for amplifying a DNA segment, producing millions of copies of a DNA sequence.
2. In this method, PCR is performed after reverse transcription.	2. The three phases are denaturation, annealing, and extension.
3. A single-stranded RNA molecule serves as the reverse transcription template, whereas a single-strand DNA molecule serves as the PCR template	3. The template is a double-stranded DNA molecule.
4. Enzymes such as reverse transcriptase and DNA polymerase are used.	4. The enzyme in this approach is DNA polymerase.
5. For reverse transcription, a reverse primer is needed.	5. Forward and reverse primers are utilised in this approach.
6. It is a more sensitive technique.	6. It is less sensitive in comparison.
7. It is used to detect gene expression.	7. It is employed in gene functional analysis, hereditary disease diagnosis, and monitoring, DNA cloning, DNA sequencing, and ancient DNA amplification.