Question

What is the direction of synthesis of DNA by DNA Polymerase?

• A. 3' to 5'
• B. 5' to 3'
• C. No set direction for DNA Polymerase
• D. Direction is decided by the Ori sequence

Answer 1

The correct option is B 5' to 3'

DNA Polymerase always adds nucleotide to the new strand from the 3' end. Therefore the direction of synthesis of DNA is said to be 5' to 3'.