Question

What is the function of DNA ligase in the formation of recombinant DNA?

• A. It catalyzes the formation of phosphodiester bonds between two broken ends of DNA
• B. It utilizes two ATP molecules.
• C. It generally combines two DNA molecules with sticky ends.
• D. All of the above

Answer 1

The correct option is D All of the above

The function of DNA ligase is to join together two DNA fragments by catalysing the formation of phosphodiester bonds. Each bond formation utilizes two ATP molecules. Distinct DNA ligases are employed for combining DNA with sticky ends and blunt ends respectively. So, the correct answer is ‘All of the above’