Question

What is the function of DNA polymerase I during DNA replication?

Answer 1

DNA polymerase I:

1. DNA polymerase I is a DNA polymerase encoded by the pol I gene and is involved in prokaryotic DNA replication.
2. DNA polymerase I is involved in removing RNA primers in the processing of DNA after replication.
3. It has three activities that is polymerase, $3\text{'}\to 5\text{'}exonuclease$ and $5\text{'}\to 3\text{'}exonuclease$

The function of DNA polymerase I:

1. DNA polymerase I also fill in the resulting gaps by synthesizing DNA, beginning at the $3\text{'}$ end of the neighboring Okazaki fragment.
2. In addition to that, it also possesses $3`\to 5`$exonuclease activity that aims at proofreading the DNA sequence that has also been replaced by RNA primer.
3. And this enzyme removes the ribonucleotides one at a time from the $5\text{'}$ end of the primer $(5\text{'}\to 3\text{'}exonuclease)$
4. Pol I is used to repair damaged DNA and is also used in the lab to prepare radiolabeled DNA.