It is a carboxylase enzyme that catalyzes the binding of 1 carbon dioxide molecule to the 3-C compound phosphoenolpyruvate, resulting in the creation of the four-carbon complex oxaloacetate.
It is the first enzyme in the Hatch Slack pathway's carbon fixing or C4.
It catalyzes the conversion of phosphoenolpyruvate (PEP) to the 4C acid, oxaloacetic acid (OAA), by adding CO2.