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Question

What is the function of primers in PCR reaction?


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Solution

PCR:

  1. The polymerase Chain reaction is a molecular biology technique that amplifies a particular gene segment.
  2. PCR primers are short fragments of single-stranded DNA, typically approximately 202020 nucleotides in length.
  3. Each PCR reaction uses two primers designed to be adjacent to the target region (the region to be copied).
  4. That is, at the end of the region to be copied, a sequence is given that binds to the opposite strand of the template DNA.
  5. Primers bind to the template via complementary base pairs.
  6. Like other DNA polymerases, Taq polymerase can only make DNA given a primer, which is a short nucleotide sequence that provides a starting point for DNA synthesis.

Thus, in the PCR reaction, the experimenter determines the region of DNA that is copied or amplified by the primer of his choice.

The figure below shows three steps A,B,C of polymerase chain reaction PCR.  Select the option giving correct identification together with what it  represents?

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