Question

What is the principle of chromatography?

Answer 1

It is defined as the process of separation of the individual components of a mixture based on their relative affinities towards stationary and mobile phases.

• Principle: The samples are subjected to flow by mobile liquid onto or through the stable stationary phase.
• This technique was originally used to separate a mixture into its chemical components based on colour but is now used more widely.
• The principle behind chromatography is the capacity of a chemical to be adsorbed on a medium. The rates of adsorption of different chemicals on a medium vary.