Question

What is the recognition site for EcoRI?

Answer 1

EcoRI:

1. EcoRI is a restriction enzyme, often known as a restriction endonuclease.
2. It severs the DNA double helix at a precise point.
3. This restriction enzyme was discovered in E.coli.
4. These restriction enzymes get their names from the species from which they were initially isolated.

The recognition site for EcoRI:

1. It is a type II restriction enzyme that cuts only at the restriction site with the 5'-GAATTC-3' sequence.
2. It chops the DNA at a specified location, resulting in sticky ends.
3. EcoRI has a palindrome recognition site that cuts DNA following G, creating sticky ends with AATT.
4. It is a six-nucleotide sequence with GAATTC in the 5' to 3' direction and CTTAAG in the 3' to 5' direction on the complementary strand.