Question

What is the role of Calcium Chloride in the heat shock method of transformation?

• A. It give positive charge to the cell wall
• B. It creates the pores of the cell membrane
• C. It help the DNA to move into the cell
• D. All of the above

Answer 1

The correct option is A It give positive charge to the cell wall

Calcium Chloride imparts a positive charge to the cell wall. Since DNA is negatively charged molecule, the positive charge on the cell wall imparted by calcium chloride helps the DNA to get attracted towards the cell, and hence ensures a higher efficiency of successful transformants.