Question

What is the role of EtBr in recombinant DNA technology?

• A. It separates the DNA from the gel matrix
• B. It helps the DNA move through the gel matrix
• C. It sticks to DNA and fluoresces under UV rays
• D. It sticks to DNA and imparts a deep red color

Answer 1

The correct option is C It sticks to DNA and fluoresces under UV rays

EtBr or Ethidium Bromide has the property of fluorescence. When added into a solution containing DNA, it percolates between the double helix of DNA. Therefore it moves along with the DNA in the gel matrix. Since it fluoresces under UV light, it helps us identify the desired DNA fragment in the gel matrix