What is the role of the thermocycler in PCR?
PCR or polymerase chain reaction:
Role of the thermocycler in PCR:
What is primers? Mention its role and the number of primers required in each cycle of PCR. Give the characteristic feature and source organism of the DNA polymerase used in PCR.
Match the lists and find the correct option:
III(a) Colony hybridisation1. Transfer of recombinant DNA into host cell(b) Gel electrophoresis2. Selection of cells containing the desired gene(c) Gradient centrifugation3. Separation of DNA fragments(d) Polymerase chain reaction4. Purification of DNA 5. Gene cloning in thermocycler
Mention the number of primers required in each cycle of polymerase chain reaction. Write the role of primers and DNA polymerase in PCR.