Question

What is the source of restriction enzymes?

Answer 1

Restriction enzyme:

1. A restriction enzyme is a type of protein that can cut DNA at a specific place by recognizing a certain sequence of nucleotides.
2. In bacteria and other prokaryotic species, restriction enzymes are found. Restriction endonucleases are another name for restriction enzymes.
3. These enzymes locate particular DNA sequences and attach to them. Restrictions sites are these websites.
4. Numerous restriction enzymes are found in bacteria.
5. More than 900 restriction enzymes have been identified from 230 different bacterial strains.
6. Only one or a small number of restriction sites can be recognized or identified by each restriction enzyme.

The two restriction enzymes mentioned are EcoRI and SmaI.

1. E. coli RY 13 bacteria are the source of the EcoRI restriction enzyme. Two sticky ends are produced by EcoRI's staggered DNA cut.
2. Serratia marcescens is the source of the SmaI restriction enzyme. SmaI cuts DNA in a straight line, leaving two blunt ends.