Question

What is tissue culture? Describe the methodology of tissue culture.

Answer 1

• Tissue culture is a method of biological research in which fragments of tissue from an animal or plant are transferred to an artificial environment in which they can continue to survive and function. The cultured tissue may consist of a single cell, a population of cells, or a whole or part of an organ. Cells in culture may multiply, change size, form, or function. They can exhibit specialized activity or interact with other cells.

Methodology for a plant tissue culture:

• Plant tissue culture is performed under aseptic conditions. Living plant materials are obtained as explants. Surface sterilization of starting material (explants) in chemical solutions (alcohol and sodium hypochlorite) is required. Explants are then usually placed on the surface of a solid culture medium or in a liquid medium.
• Solid and liquid media are generally composed of inorganic salts and organic nutrients, vitamins and plant hormones.

As the cultures grow, there is the formation of callus which is an undifferentiated mass of cells.

• The callus divides and the cells are transferred to new media to allow the growth of the cells. The plant hormones can be added which can promote shoot and root formation. These plants are cultured in nurseries and greenhouses. Later, the plants are transferred to the fields.