Question

What is totipotency? Describe the important method of tissue culture with the help of diagram.

Answer 1

Totipotency is the ability of a cell to form the complete organism. A plant cell can form the complete plant because of totipotency.

Tissue culture works on the basis of totipotency. It is the in vitro culture of Plant cell, tissues and even organs. Tissue culture involves following steps:

1. Preparation of nutrient medium

Most common nutrient medium used for plant tissue culture is MS medium.

2. Explant is taken and put on nutrient medium

Explant is any excised part of the plant which can regenerate into a complete plant.

3. Optimum conditions are provide to the cultures.

4. Proper aseptic conditions are made to avoid contamination.

5. Cultures when grown can further be sub cultured.